TRICHOMONAS MODIFIED CPLM MEDIUM BASE (MODIFIED CPLM MEDIUM BASE)

Trichomonas is a protozoan, similar to bacteria. Trichomonas vaginalis is a causative agent of trichomonalis, the most common protozoan infection in humans. It can infect the vagina and urethra in women, and sometimes the prostate gland in men. The duration of survival of T. vaginalis in transport medium is fairly limited. The organisms die rapidly when dried on a swab; an alternative approach is to place the loaded swab promptly into a tube of Trichomonas Culture Medium supplemented with horse serum, penicillin and streptomycin. Media for cultivation of T. vaginalis basically provide essential salts, nutrients, reducing agents and antibiotics to inhibit bacterial growth in the absence or in low concentration of oxygen. This medium was further modified without agar and methylene blue. Under strictly anaerobic conditions, this medium supports growth from a single protozoan. Under aerobic conditions, massive inocula are required. T. vaginalis is an anaerobe and contains no catalase.

for cultivation of Trichomonas species