TRYPTONE DEXTROSE AGAR

Tryptone Agar was developed by Vera for the accurate differentiation and identification of aerobes and anaerobes by means of motility and fermentation reactions. It is recommended for Clostridia, Bacillus species, Micrococci, enteric bacilli and other non-fastidious organisms.
Small amount of agar renders it suitable for study of motility. Acid produced do not readily get dispersed throughout the medium and hence positive reaction can be more quickly determined in this medium than in liquid medium. This is also an excellent medium for the maintenance for both – aerobic and anaerobic cultures. Viability in this medium is greater than in any other broth medium or slant culture. Organisms capable of utilizing dextrose, ferment dextrose and produce acidic conditions in the medium. This acidity is detected by the pH indicator bromothymol blue which changes from blue to yellow under acidic conditions.

for studying motility and fermentation of dextrose by aerobes and anaerobes