TRYPTONE GLUCOSE YEAST EXTRACT BROTH (STANDARD METHOD BROTH) (VEG.)

These media are prepared by using Veg hydrolysate which is free from BSE/TSE risks associated with animal based peptones. These media are the modification of Tryptone Glucose Yeast Extract Agar/Broth which was originally developed by Bowers and Hucker. These media serves the same purpose as the conventional media which are recommended for the cultivation and enumeration of bacteria in air, water, milk and dairy products. Also these media can be used for various studies of thermophilic bacteria in milk, influence of incubation temperature etc. Tryptone Glucose Extract Veg Agar/Broth like the conventional medium are used for the bacteriological plate count of milk and dairy products and for the enumeration of microorganisms during microbiological examination of food materials by MPN technique.
This medium can be used for pour plate technique. Usually 1 ml of appropriate dilution of the test sample are pipetted in to sterile pertiplates and molten, Tryptone Glucose Yeast Extract Veg Agar is added followed by gentle mixing to mix the sample well and incubate the plates for 48 ?72 hours at 32 ? 35?C.

for enumeration of microorganisms from foods by MPN technique