TRYPTONE PHOSPHATE BROTH

Certain biotypes of Escherichia coli are etiological agents of gastrointestinal illness in humans and several mammals. These enteric diseases are characterized by fever, vomitting and prominent and watery diarrhea, usually with mucus but not blood. Enteropathogenic E.coli serotypes have been shown to be important causes of infantile diarrhoea. Tryptone Phosphate Broth is formulated as recommended by APHA for the enrichment of Enteropathogenic E. coli. . Refrigerate perishable material less than 1 day to avoid damages to the bacteria. Aseptically weigh 25 grams test portion into 225 ml Brain Heart Infusion Broth. Agitate gently, and incubate for 2 hours at 35?C. After incubation, streak loopful on MacConkey Agar and on EMB Agar. Incubate at 35?C for 2 hours. Pour the supernatant into 250 ml double strength Tryptone Phosphate Broth. Incubate at 44?C for 20?2 hours. Subsequently streak on EMB Agar and MacConkey Agar. Tryptone Phosphate Broth helps to enrich the stressed bacteria, if present.

for cultivation of enteropathogenic Escherichia coli from foods