TRYPTOSE AGAR, W/ THIAMINE HCL

Huddleson used Tryptose media for the isolation of Brucella species from man. Tryptose containing media, rather than the conventionally used meat infusion media have been used for the enumeration and isolation of Brucella species. Addition of thiamine to tryptose media enhanced the recovery of Brucella species especially Brucella suis. These media can be used as general purpose media for cultivation of wide variety of organisms. It can also be supplemented with defibrinated blood (sheep, horse) to prepare blood agar for the isolation of fastidious organisms like Brucella. Tryptose Agar with thiamine HCl is recommended by APHA and Diagnostic Procedures and Reagents for the isolation and cultivation of Brucella species and also Streptococci, meningococci, pneumococci and other pathogenic bacteria.

for the isolation, cultivation and di?erentiation of fastidious microorganisms in an infusion free medium