TRYPTOSE BROTH

Huddleson used Tryptose broth media for the isolation of Brucella species from man. Tryptose containing media, rather than the conventionally used meat infusion media have been used for the enumeration and isolation of Brucella species.
Tryptose Broth is also recommended by APHA and FDA. This medium can be used as general purpose media for cultivation of wide variety of organisms. It can also be supplemented with defibrinated blood (sheep, horse) to prepare blood containing medium for the isolation of fastidious organisms like Brucella. Tryptose Broth can be supplemented with 0.1% agar for the cultivation of anaerobes.

for cultivation of Brucella species microorganisms and determining the haemolytic reactions