TRYPTOSE BROTH, W/ THIAMINE HCI

Huddleson used Tryptose media for the isolation of Brucella species from man. Tryptose containing media, rather than the conventionally used meat infusion media have been used for the enumeration and isolation of Brucella species. Addition of thiamine to tryptose media enhanced the recovery of Brucella species especially Brucella suis.
This medium can be used as general purpose medium for cultivation of wide variety of organisms. It can also be supplemented with defibrinated blood (sheep, horse) to prepare blood containing medium for the isolation of fastidious organisms like Brucella. Tryptose Broth with thiamine HCl is recommended by APHA and Diagnostic Procedures and Reagents for the isolation and cultivation of Brucella species and also Streptococci, Meningococci, Pneumococci and other pathogenic bacteria.

for the cultivation and di?erentiation of fastidious microorganisms in an infusion free medium