TRYPTOSE SERUM BROTH BASE (MODIFIED NEWINGS TRYPTOSE BROTH BASE)

Tryptose Serum Broth Base demonstrated by Newing & McLeod, 1958 was later modified by Gourlay (1964). This medium is as described by Davies. This medium is recommended for the cultivation of Mycoplasma. The medium ingredients and all the supplements should be free of any toxic substances even in small amounts. Many Mycoplasma require serum for their good growth and also presence of antibiotic is necessary to prevent the growth of contaminating organisms. Mostly the Mycoplasma species are aerobic or facultatively anaerobic but some are microaerophilic. Few are anaerobic saprophytic Mycoplasma which grow best at 22-35?C while pathogenic strains grow at 35?C.

for routine identi?cation of Mycoplasma species