VAGINALIS AGAR BASE

Gardnerella vaginalis is a facultatively anaerobic gram-variable rod. It has been demonstrated to cause a wide variety of infections; however, it is most commonly recognized for its role as one of the organisms responsible for bacterial vaginosis (BV). BV is the most common cause of vaginitis and the most common infection encountered in the outpatient gynaecological setting. Originally Ellner et al developed a blood agar namely Columbia Agar for rapid growth of the haemolytic organisms with improved pigmentation and defined haemolytic reactions. Greenwood et al further modified this medium by increasing the peptone concentration and used human blood instead of sheep blood for the isolation and differentiation of G. vaginalis based on beta haemolysis. Vaginalis Agar Base is used for the isolation of G. vaginalis from vaginal discharges.
Typical colonies of G. vaginalis appear small and white coloured. This medium is recommended for determin ation of haemolytic reaction of G. vaginalis and not for other microorganisms. If the specimen is suspected to contain streptococci or other haemolytic microorganisms, then a Soyabean Casein Digest Agar (with 5% v/v sheep blood) plate should be inoculated parallel to this medium to ensure the haemolytic reaction.

for isolation and di?erentiation of Gardenerella vaginalis from clinical samples