VITAMIN FREE YEAST BASE

Yeasts are unicellular, eukaryotic, budding cells that are generally round, oval or elongated in shape and are considered as opportunistic pathogens. They multiply principally by the production of blastoconidia (buds). Yeast colonies are moist and creamy or glabrous to membranous in texture. Moulds are microscopic, plant-like organisms, composed of long filaments called hyphae. Both are widely distributed in soil, water and air. Cultivation of yeasts and moulds becomes important in fermentation studies where they are generally used as starter cultures. Vitamin Free Yeast Base is recommended for classification of yeasts based on vitamin requirement. It contains all essential nutrients and necessary inorganic salts for the cultivation of yeasts. Use a highly diluted inoculum and incubate the tubes for 7 days at 25-28?C, since with the inoculum, vitamins may also be transported. Yeast themselves are also able to carry traces of vitamins, and therefore a second inoculation in Vitamin Free Yeast Base must be performed following the same procedure as for the first inoculation. Then incubate at 25-28?C for 7 days.

for studying vitamin requirements of yeasts